Isolation of Mesenchymal Stromal Cells from Human Exfoliated Deciduous Teeth
DOI:
https://doi.org/10.52340/GBMN.2025.01.01.133Keywords:
Mesenchymal stromal cells (MSCs), phenotypic characterization, stem cells derived from human exfoliated deciduous teeth (SHED)Abstract
Background: Mesenchymal stromal cells (MSCs) have been intensively studied for clinical applications over the last few decades. Their potential for tissue regeneration has driven high interest in these cells. One source of MSCs is human dental pulp (DPSCs, SHED). Recently, mesenchymal stromal cells derived from human exfoliated deciduous teeth (SHED) have attracted particular attention in part due to their high expression of classic pluripotency/embryonic antigens. Objectives: There are various practical methods for processing, deriving, and proliferating SHED that are being investigated for their use in regenerative medicine. Methods: In this work, we demonstrate the establishment of a culture of mesenchymal stromal cells from primary tooth pulp using the explant culture method. Results: Flow cytometric phenotypic characterization revealed robust expression of mesenchymal surface markers CD90, CD73, and CD105, accompanied by negligible levels of hematopoietic markers CD45 and CD34. These findings suggest that the isolated cells derived from human exfoliated deciduous tooth pulp possess a mesenchymal stromal phenotype. Conclusions: This optimized protocol may facilitate the widespread cultivation of mesenchymal stromal cells in the laboratory for further use in preclinical research.References
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