Semiorthotopic Model for Stem Cell-Based Pulp Regeneration: Foundation for Preclinical Research
DOI:
https://doi.org/10.52340/GBMN.2025.01.01.136Keywords:
Regenerative endodontics, semiorthotopic model, tooth-slice modelAbstract
Background: Advances in biotechnology and tissue engineering have laid the foundation for the development of new strategies in regenerative endodontics. In recent years, the regeneration of dental tissues, particularly the restoration of the dental pulp, has emerged as a dynamic and promising field of research. Objectives: Several preclinical and experimental models are used to investigate the mechanisms of dental pulp regeneration and to evaluate the efficacy of newly developed bioengineered materials. In the present study, we describe a method for constructing a bioengineered tooth-slice model and assess the performance of its incorporated material using a semiorthotopic experimental design. Methods: For the preparation of tooth slices, four mandibular incisor teeth (n = 4) from sheep were used. Following longitudinal sectioning, two-thirds of the pulp tissue were amputated, and the remaining canal space was filled with mesenchymal stromal cells derived from human exfoliated deciduous teeth (SHED) loaded onto microcarriers (CultiSpher®) and embedded within a fibrinogen–thrombin gel. The resulting bioengineered construct was implanted subcutaneously in the dorsal region of rats. Histological evaluation of the bioengineered material’s effect was performed at 7 and 28 days. Results: The bioengineered material introduced into the tooth slice, in conjunction with the remaining pulp tissue, promoted the formation of pulp-like and dentin- like structures. Conclusions: The development of a bioengineering algorithm represents a multistage, technologically demanding process that requires the iterative optimization of cellular, scaffold, and matrix components to achieve a functional synergistic effect. The present study demonstrated the distinct odontogenic and regenerative potential of the formulated bioengineered material, providing a robust basis for subsequent preclinical validation and translational application in regenerative endodontics.References
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